C. elegans: Methods and Applications by Kevin Strange (English) Paperback Book

Description: C. elegans by Kevin Strange Molecular biology has driven a powerful reductionist, or "molecule-c- tric," approach to biological research in the last half of the 20th century. Systems biology is integrative and seeks to understand and predict the behavior or "emergent" properties of complex, multicomponent biological processes. FORMAT Paperback LANGUAGE English CONDITION Brand New Publisher Description Nonmammalian model organisms have become a cornerstone of systems biology research. Like the Rosetta Stone, which enabled modern scholars to decode ancient Egyptian hieroglyphics, model organisms enable biologists to decipher the genetic code underlying the complex physiological processes common to all life. C. elegans provides a particularly striking example of the experimental utility of model organisms. Genetic, molecular and systems biological characterization of this organism is greatly facilitated by its short life cycle and high rate reproduction, and by the ease with which it can be cultured in the laboratory. C. elegans also has a fully sequenced and well-annotated genome, which is assembled in readily accessible public databases along with virtually all other biological data on this organism. C. elegans: Methods and Applications aims to enhance the readily available methodologies for the study of C. elegans by providing overviews and concise, step-by-step descriptions of the most state-of-the-art methods currently being utilized in the field.Some of the methods presented include forward and reverse genetic analysis, data mining and comparative genomics strategies, electron and fluorescence microscopy methods, automated imaging methods for worm behavioral analysis, functional genomics strategies, and cutting-edge methods for physiological analyses. This comprehensive collection of methods, written by experts in the field, endeavors to serve as a roadmap for researchers, illustrating the type or research that is possible with C. elegans, and illuminating its potential as a vehicle for future discovery. Table of Contents An Overview of C. elegans Biology.- Comparative Genomics in C. elegans, C. briggsae, and Other Caenorhabditis Species.- WormBase.- C. elegans Deletion Mutant Screening.- Insertional Mutagenesis in C. elegans Using the Drosophila Transposon Mos1.- Single-Nucleotide Polymorphism Mapping.- Creation of Transgenic Lines Using Microparticle Bombardment Methods.- Construction of Plasmids for RNA Interference and In Vitro Transcription of Double-Stranded RNA.- Delivery Methods for RNA Interference in C. elegans.- Functional Genomic Approaches in C. elegans.- Assays for Toxicity Studies in C. elegans With Bt Crystal Proteins.- Fluorescent Reporter Methods.- Electrophysiological Analysis of Neuronal and Muscle Function in C. elegans.- Sperm and Oocyte Isolation Methods for Biochemical and Proteomic Analysis.- Preservation of C. elegans Tissue Via High-Pressure Freezing and Freeze-Substitution for Ultrastructural Analysis and Immunocytochemistry.- Intracellular pH Measurements In Vivo Using Green Fluorescent Protein Variants.- Automated Imaging of C. elegans Behavior.- Intracellular Ca2+ Imaging in C. elegans.- In Vitro Culture of C. elegans Somatic Cells.- Techniques for Analysis, Sorting, and Dispensing of C. elegans on the COPAS™ Flow-Sorting System. Long Description Molecular biology has driven a powerful reductionist, or "molecule-c- tric," approach to biological research in the last half of the 20th century. Red- tionism is the attempt to explain complex phenomena by defining the functional properties of the individual components of the system. Bloom (1) has referred to the post-genome sequencing era as the end of "na Feature Includes supplementary material: sn.pub/extras Details ISBN161737668X Publisher Humana Press Inc. Series Methods in Molecular Biology Year 2010 Edition 1st ISBN-10 161737668X ISBN-13 9781617376689 Format Paperback Imprint Humana Press Inc. Place of Publication Totowa, NJ Country of Publication United States Edited by Kevin Strange DEWEY 576.5 Short Title C ELEGANS Language English Media Book Series Number 351 Subtitle Methods and Applications Pages 292 Illustrations 42 Illustrations, black and white; XII, 292 p. 42 illus. Publication Date 2010-12-01 AU Release Date 2010-12-01 NZ Release Date 2010-12-01 US Release Date 2010-12-01 UK Release Date 2010-12-01 Author Kevin Strange Edition Description Softcover reprint of hardcover 1st ed. 2006 Alternative 9781588295972 Audience Professional & Vocational We've got this At The Nile, if you're looking for it, we've got it. With fast shipping, low prices, friendly service and well over a million items - you're bound to find what you want, at a price you'll love! TheNile_Item_ID:96237206;

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